Journal: Small (Weinheim an Der Bergstrasse, Germany)

Article Title: Multifunctional Co‐Delivery Systems with Downregulation of the Novel Target PIM1 in Macrophages to Ameliorate TF‐Mediated Coagulopathy in Sepsis

doi: 10.1002/smll.202412688

Figure Lengend Snippet: PIM1 inhibitor SMI‐4a protects septic mice against coagulation activation and sepsis‐induced acute lung injury. A) Schematic representation of animal experimental procedures. B) Representative western blot membranes and corresponding densitometric analyses of (C) PIM1 in lung tissue (n = 6/group). D) mRNA levels of PIM1 in murine lung tissues (n = 5/group). E) Platelet count in mice (n = 4/group). F–H) Plasma levels of coagulation‐related factors in mice plasma by ELISA, including (F) TAT, (G) D‐dimer, and (H) fibrinogen (n = 4/group). I) The lung sections were subjected to hematoxylin and eosin (HE) staining, F4/80, fibrin, and PIM1 immunohistochemical analysis (n = 4/group; scale bar:50 µm). J) Representative western blot membranes and corresponding densitometric analyses of (K) TF, (L) PAI‐1, (M) Thrombin in lung tissue (n = 6/group). N) mRNA levels of TF in murine lung tissues (n = 5/group). O–Q) mRNA levels of (O) IL‐1β, (P) IL‐6, and (Q) TNF‐ɑ in murine lung tissues (n = 5/group). R) Survival curves of mice in all groups (n = 10/group). Each bar represents the mean ± SD. Statistical analysis for three or more groups was carried out using one‐way ANOVA (C‐H and K‐Q). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The concentrations of PIM1 (CSB‐ E11825 h, Cusabio, China) in human plasma and TAT (CSB‐ E08433 m, Cusabio, China), Fbg (CSB‐ E08202 m, Cusabio, China), and D2D (CSB‐ E13584 m, Cusabio, China) in mouse plasma were assessed using ELISA kits according to the guidelines outlined in the respective ELISA kits.

Techniques: Coagulation, Activation Assay, Western Blot, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Staining, Immunohistochemical staining

Journal: bioRxiv

Article Title: Circulatory neutrophils exhibit enhanced neutrophil extracellular trap formation in early puerperium: NETs at the nexus of thrombosis and immunity?

doi: 10.1101/2021.10.18.459827

Figure Lengend Snippet: ( a .) Immunohistochemical staining for TF (green), and DNA counterstain with DAPI (blue) after a 3-hour in vitro culture. Magnification: 20x; Scale bars: 50μm. ( b .) Platelet differential counts in healthy non-pregnant blood donors, donors during pregnancy and 48 hours postpartum. ( c .) Comparative analysis between healthy donor controls, donors during gestation and postpartum, concerning the platelet morphology and activation indices mean platelet volume (MPV) and platelet distribution width (PDW). ( d .) TAT complexes concentration in citrate plasma from healthy donor controls, donors during pregnancy and postpartum, determined by ELISA. ( e .) Spearman correlation between TAT complexes concentration and NET-associated MPO/DNA complexes. ( f .) D-dimer levels in citrate plasma from healthy donor controls, donors during gestation and postpartum, determined by ELISA. P: pregnancy; PD: post-delivery. Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (one way ANOVA followed by Bonferroni’s multiple comparison post-test). All experiments were performed at least 6 times with consistent results.

Article Snippet: The concentrations of thrombin-antithrombin (TAT) complexes and D-dimers were measured by sandwich ELISA, utilizing the human TAT Complexes ELISA Kit (Assaypro) and the Imuclone D-Dimer ELISA Kit (American Diagnostica) respectively.

Techniques: Immunohistochemical staining, Staining, In Vitro, Activation Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay